primary antibody human hsp47  (R&D Systems)

Journal: Current protocols

Article Title: Simple, Fast, and Efficient Method for Derivation of Dermal Fibroblasts From Skin Biopsies.

doi: 10.1002/cpz1.714

Figure Lengend Snippet: Figure 3 Skin biopsy–derived cells express fibroblast-specific markers. Upon fixation with 4% PFA, cells were stained with specific antibodies. (A) Anti-vimentin (in red) and anti-Oregon Green 488 Phalloidin (F-actin, in green) staining. (B) Anti-Hsp47 antibody (in green) staining. Nuclei were detected by DAPI (in blue) staining.

Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Table 1 List of Antibodies Used for Characterizing Fibroblasts by Immunocytochemistry Species Company Cat # Dilution Primary antibody Human Hsp47 (Serpin H1) Antibody Monoclonal mouse IgG2B R&D Systems MAB91661SP 1:200 to 1:500 Oregon Green 488 Phalloidin (F-actin) (conjugated) Amanita phalloides mushroom Thermo Fisher Scientific O7466 1:50 Vimentin mouse McAb Mouse IgG1 Proteintech 60330-1-Ig 1:500 Secondary antibody F(ab’)2-Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 546 Goat anti-Mouse Thermo Fisher Scientific A-11018 1:1000 F(ab’)2-Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Goat anti-Mouse Thermo Fisher Scientific A-11017 1:1000 dermal fibroblasts can be expanded by passaging with trypsin (in our hands, fibroblasts still showed the ability to divide up to passage five, but we did not try to passage more than P5).

Techniques: Derivative Assay, Staining